| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 421 | Escherichia marmotae | AACCGTCACAACCGCTGA | TTTCCACCACGACGGGTT | 59.50 | 59.09 | 191 | 55 |
100.00%
|
100.00%
|
| Primer 422 | Escherichia marmotae | ACGAGGCTAAAGCGCAGA | ATGCATAAGCCGTCGCCT | 59.03 | 59.49 | 288 | 55 |
100.00%
|
100.00%
|
| Primer 423 | Escherichia marmotae | TTCGCTGCTGGAAGTGCT | CCCGACAATTTCCAGCCCT | 59.57 | 60.00 | 220 | 55 |
100.00%
|
100.00%
|
| Primer 424 | Escherichia marmotae | TGTGGAAACGCTGGCAGT | GCATCAACACCACGCGTT | 59.81 | 59.36 | 231 | 55 |
100.00%
|
100.00%
|
| Primer 425 | Escherichia marmotae | AGAGTTTCGCCAGGTCTGC | AACACCCAATCCGCTGCA | 60.00 | 59.88 | 216 | 55 |
100.00%
|
100.00%
|
| Primer 426 | Escherichia marmotae | TGCGCTGGGTTGATCCAA | GTGGTTTACGCTGCGCAA | 59.57 | 59.37 | 174 | 55 |
100.00%
|
100.00%
|
| Primer 427 | Escherichia marmotae | TTGCGCTGGGTTGATCCA | GTGGTTTACGCTGCGCAA | 59.57 | 59.37 | 175 | 55 |
100.00%
|
100.00%
|
| Primer 428 | Escherichia marmotae | GGCACCCTGTACACCATGA | GAACGCCTGCAGCAACAA | 59.32 | 59.28 | 192 | 55 |
100.00%
|
100.00%
|
| Primer 429 | Escherichia marmotae | TACAGGCCTCTTCCGAGCT | AGCGCATCATGCCCTGTT | 60.00 | 60.04 | 189 | 55 |
100.00%
|
100.00%
|
| Primer 430 | Escherichia marmotae | ATCGAAACGCAGCAACGG | AAATGCCTGGGTGCCCAA | 59.14 | 59.80 | 186 | 55 |
100.00%
|
100.00%
|